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3rd order polynomial fit of the fret index  (MathWorks Inc)


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    MathWorks Inc 3rd order polynomial fit of the fret index
    <t>FRET</t> analysis of intracellular calcium level with TN-XXL in HEK293T cells. Cells were perfused with 40 mM glucose (A-F). Open bars with gray columns indicate the bolus of external glucose during perfusion with glucose-free Hanks’ balanced buffer. Quantitative data were derived by pixel-by-pixel integration of the ratiometric images. The fluorescence intensity in arbitrary units (A.U.) for individual eCFP (ET470/24m) and Citrine (ET535/30m) emission channels were monitored with eCFP excitation (ET430/24x) and the <t>FRET</t> <t>index</t> for Venus and eCYP was determined (Y-axis corresponds to sensitized fluorescence Fc (background and bleed-through corrected using Citrine excitation (ET500/20x)) normalized to donor emission; note that peak intensities were used and that only the short wavelength emission peak of eCFP was considered for donor emission). FRET images were acquired every 5 sec and cytosolic calcium levels were analyzed. HEK293T cells were preincubated with 50 μM gadolinium (30 min) (B), 10 μM nifedipine (30 min) (C), 1 μM thapsigargin (15 min; D, 30 min; E) or no calcium (30 min) (F) without glucose. When steady-state calcium values were reached, a bolus of 40 mM glucose was added for 2 min. Data are shown as mean ± SD (n = 5-12).
    3rd Order Polynomial Fit Of The Fret Index, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3rd order polynomial fit of the fret index/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    3rd order polynomial fit of the fret index - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Osmotic induction of calcium accumulation in human embryonic kidney cells detected with a high sensitivity FRET calcium sensor"

    Article Title: Osmotic induction of calcium accumulation in human embryonic kidney cells detected with a high sensitivity FRET calcium sensor

    Journal: Cell calcium

    doi: 10.1016/j.ceca.2009.06.003

    FRET analysis of intracellular calcium level with TN-XXL in HEK293T cells. Cells were perfused with 40 mM glucose (A-F). Open bars with gray columns indicate the bolus of external glucose during perfusion with glucose-free Hanks’ balanced buffer. Quantitative data were derived by pixel-by-pixel integration of the ratiometric images. The fluorescence intensity in arbitrary units (A.U.) for individual eCFP (ET470/24m) and Citrine (ET535/30m) emission channels were monitored with eCFP excitation (ET430/24x) and the FRET index for Venus and eCYP was determined (Y-axis corresponds to sensitized fluorescence Fc (background and bleed-through corrected using Citrine excitation (ET500/20x)) normalized to donor emission; note that peak intensities were used and that only the short wavelength emission peak of eCFP was considered for donor emission). FRET images were acquired every 5 sec and cytosolic calcium levels were analyzed. HEK293T cells were preincubated with 50 μM gadolinium (30 min) (B), 10 μM nifedipine (30 min) (C), 1 μM thapsigargin (15 min; D, 30 min; E) or no calcium (30 min) (F) without glucose. When steady-state calcium values were reached, a bolus of 40 mM glucose was added for 2 min. Data are shown as mean ± SD (n = 5-12).
    Figure Legend Snippet: FRET analysis of intracellular calcium level with TN-XXL in HEK293T cells. Cells were perfused with 40 mM glucose (A-F). Open bars with gray columns indicate the bolus of external glucose during perfusion with glucose-free Hanks’ balanced buffer. Quantitative data were derived by pixel-by-pixel integration of the ratiometric images. The fluorescence intensity in arbitrary units (A.U.) for individual eCFP (ET470/24m) and Citrine (ET535/30m) emission channels were monitored with eCFP excitation (ET430/24x) and the FRET index for Venus and eCYP was determined (Y-axis corresponds to sensitized fluorescence Fc (background and bleed-through corrected using Citrine excitation (ET500/20x)) normalized to donor emission; note that peak intensities were used and that only the short wavelength emission peak of eCFP was considered for donor emission). FRET images were acquired every 5 sec and cytosolic calcium levels were analyzed. HEK293T cells were preincubated with 50 μM gadolinium (30 min) (B), 10 μM nifedipine (30 min) (C), 1 μM thapsigargin (15 min; D, 30 min; E) or no calcium (30 min) (F) without glucose. When steady-state calcium values were reached, a bolus of 40 mM glucose was added for 2 min. Data are shown as mean ± SD (n = 5-12).

    Techniques Used: Derivative Assay, Fluorescence



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    3rd order polynomial fit of the fret index  (MathWorks Inc)
    90
    MathWorks Inc 3rd order polynomial fit of the fret index
    <t>FRET</t> analysis of intracellular calcium level with TN-XXL in HEK293T cells. Cells were perfused with 40 mM glucose (A-F). Open bars with gray columns indicate the bolus of external glucose during perfusion with glucose-free Hanks’ balanced buffer. Quantitative data were derived by pixel-by-pixel integration of the ratiometric images. The fluorescence intensity in arbitrary units (A.U.) for individual eCFP (ET470/24m) and Citrine (ET535/30m) emission channels were monitored with eCFP excitation (ET430/24x) and the <t>FRET</t> <t>index</t> for Venus and eCYP was determined (Y-axis corresponds to sensitized fluorescence Fc (background and bleed-through corrected using Citrine excitation (ET500/20x)) normalized to donor emission; note that peak intensities were used and that only the short wavelength emission peak of eCFP was considered for donor emission). FRET images were acquired every 5 sec and cytosolic calcium levels were analyzed. HEK293T cells were preincubated with 50 μM gadolinium (30 min) (B), 10 μM nifedipine (30 min) (C), 1 μM thapsigargin (15 min; D, 30 min; E) or no calcium (30 min) (F) without glucose. When steady-state calcium values were reached, a bolus of 40 mM glucose was added for 2 min. Data are shown as mean ± SD (n = 5-12).
    3rd Order Polynomial Fit Of The Fret Index, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3rd order polynomial fit of the fret index/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    3rd order polynomial fit of the fret index - by Bioz Stars, 2026-03
    90/100 stars
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    Image Search Results


    FRET analysis of intracellular calcium level with TN-XXL in HEK293T cells. Cells were perfused with 40 mM glucose (A-F). Open bars with gray columns indicate the bolus of external glucose during perfusion with glucose-free Hanks’ balanced buffer. Quantitative data were derived by pixel-by-pixel integration of the ratiometric images. The fluorescence intensity in arbitrary units (A.U.) for individual eCFP (ET470/24m) and Citrine (ET535/30m) emission channels were monitored with eCFP excitation (ET430/24x) and the FRET index for Venus and eCYP was determined (Y-axis corresponds to sensitized fluorescence Fc (background and bleed-through corrected using Citrine excitation (ET500/20x)) normalized to donor emission; note that peak intensities were used and that only the short wavelength emission peak of eCFP was considered for donor emission). FRET images were acquired every 5 sec and cytosolic calcium levels were analyzed. HEK293T cells were preincubated with 50 μM gadolinium (30 min) (B), 10 μM nifedipine (30 min) (C), 1 μM thapsigargin (15 min; D, 30 min; E) or no calcium (30 min) (F) without glucose. When steady-state calcium values were reached, a bolus of 40 mM glucose was added for 2 min. Data are shown as mean ± SD (n = 5-12).

    Journal: Cell calcium

    Article Title: Osmotic induction of calcium accumulation in human embryonic kidney cells detected with a high sensitivity FRET calcium sensor

    doi: 10.1016/j.ceca.2009.06.003

    Figure Lengend Snippet: FRET analysis of intracellular calcium level with TN-XXL in HEK293T cells. Cells were perfused with 40 mM glucose (A-F). Open bars with gray columns indicate the bolus of external glucose during perfusion with glucose-free Hanks’ balanced buffer. Quantitative data were derived by pixel-by-pixel integration of the ratiometric images. The fluorescence intensity in arbitrary units (A.U.) for individual eCFP (ET470/24m) and Citrine (ET535/30m) emission channels were monitored with eCFP excitation (ET430/24x) and the FRET index for Venus and eCYP was determined (Y-axis corresponds to sensitized fluorescence Fc (background and bleed-through corrected using Citrine excitation (ET500/20x)) normalized to donor emission; note that peak intensities were used and that only the short wavelength emission peak of eCFP was considered for donor emission). FRET images were acquired every 5 sec and cytosolic calcium levels were analyzed. HEK293T cells were preincubated with 50 μM gadolinium (30 min) (B), 10 μM nifedipine (30 min) (C), 1 μM thapsigargin (15 min; D, 30 min; E) or no calcium (30 min) (F) without glucose. When steady-state calcium values were reached, a bolus of 40 mM glucose was added for 2 min. Data are shown as mean ± SD (n = 5-12).

    Article Snippet: The baseline of the recordings was corrected using a 3 rd order polynomial fit of the FRET index measured in the absence of glucose in Matlab (the script was programmed by Dr. Oliver Schweissgut, http://www.uni-siegen.de/fb11/simtec/software/fret/ ).

    Techniques: Derivative Assay, Fluorescence